試劑一、試劑二和試劑三均需避光保存。

試劑三變藍綠色后不能再使用。

加入試劑三后必須立即混勻，否則顯色不全。

取0.1g小鼠胰腺加入1mL提取液進行勻漿研磨，取上清后按照測定步驟操作，測得計算A測定管=0.324、A對照管=0.037、A空白管=0.022、A標準管=0.677，按樣本質(zhì)量計算酶活：

取兔血清后直接按照測定步驟操作，測得計算A測定管=0.190、A對照管=0.015、A空白管=0.022、A標準管=0.677，按血液體積計算酶活：

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